Question

Cholesterol: Cholesterol oxidase:: Esters: ______________

(a) Glucose oxidase

(b) Catalase

(c) Chymotrypsin

(d) Penicillinase

This question was posed to me during an interview.

Question is from Calorimetric Biosensors topic in portion Biosensors of Enzyme Technology

Answer 1

Correct option is (c) Chymotrypsin

To explain: As cholesterol is the reactant for cholesterol oxidase which produces 53 kJ/mol heat output on the reaction completion, esters are catalyzed chymotrypsin which produces 4-16 kJ/mol of heat. Glucose oxidase, catalase and penicillinase act on glucose, hydrogen peroxide and penicillin G to produce heat which may be measured by calorimetric biosensor.