Question

Which of the following is a disadvantage of an immobilized enzyme?

(a) Immobilization process allows continuous process

(b) Immobilization mean additional cost

(c) Increase productivity

(d) Immobilization prevents loss of activity

The question was posed to me during an online interview.

This key question is from Methods of Immobilization topic in division Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

Answer 1

Correct choice is (b) Immobilization mean additional cost

For explanation I would say: The disadvantage of an immobilized enzyme is additional cost. Hence it is used only when they have sound economic, safety and process benefits over free enzymes. The advantages are as follows:

• Immobilization allows continuous process to be practicable, with a considerable saving in enzyme, labor and overhead cost.

• Productivity increased as it may be used fully at higher concentration and for a longer time.

• Immobilization prevents loss of activity as immobilized enzymes cannot be denatured.