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Which of the following was used to gas out oxygen in the dynamic method of gassing-out?

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Which of the following was used to gas out oxygen in the dynamic method of gassing-out?

(a) Growing culture

(b) Nitrogen gas

(c) Dead cells

(d) Helium

I have been asked this question during an online exam.

Enquiry is from Aeration and Agitation topic in portion Aeration and Agitation of Fermentation Technology

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The correct answer is (a) Growing culture

The explanation: The growing culture was used to gas out the oxygen in the dynamic method of gassing-out. The culture utilized all the oxygen by respiring in the fermenter for their growth and simultaneously the aeration was also stopped.

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