Question

Which of the following features is not possessed by biosensor?

(a) The biocatalyst used in the biosensor must be highly specific for the purpose of the analyses

(b) The reaction occurring in the biosensor should be as independent of such physical parameters

(c) Active site is mainly constituted by non-polar amino acids for catalysis to take place

(d) The response from the biosensors should be accurate, precise, reproducible and linear

This question was posed to me in an interview.

The doubt is from Biosensors topic in portion Biosensors of Enzyme Technology

Answer 1

Correct choice is (c) Active site is mainly constituted by non-polar amino acids for catalysis to take place

Easy explanation: Some of the features possessed by biosensors are discussed below:

• The biocatalyst used in the biosensor must be highly specific for the purpose of the analyses.

• The reaction occurring in the biosensor should be as independent of such physical parameters which allows the analysis of samples with minimal pre-treatment. Also if cofactors or coenzymes are involved, they should also be co-immobilised with the enzyme.

• The response from the biosensors should be accurate, precise, reproducible, linear over the useful analytical range and also free from electrical noise.

“Active site is mainly constituted by non-polar amino acids for catalysis to take place.” This statement is possessed by active site of the enzyme.