Question

Which of the following approach is not a key to maintaining enzyme activity?

(a) Use of additives

(b) Controlled used of covalent modification

(c) Accurate, precise, reproducible, linear and free from electrical noise

(d) Enzyme immobilization

I have been asked this question in class test.

Origin of the question is Enzyme Preparation for Sale topic in portion Enzyme Preparation and Usage of Enzyme Technology

Answer 1

The correct option is (c) Accurate, precise, reproducible, linear and free from electrical noise

To explain I would say: “The response should be accurate, precise, reproducible, linear and free from electrical noise” is a beneficial feature of biosensor. The key to maintaining enzyme activity is the maintenance of conformation which means to prevent unfolding, aggregation and changes in the covalent structure. Three approaches which are used are:

• Use of additives.

• Controlled used of covalent modification.

• Enzyme immobilization.