Question

Which of the following causes of enzyme inactivation?

(a) Filtration

(b) Loss of cofactor or coenzymes

(c) Centrifugation

(d) Crossflow microfiltration

I have been asked this question in an interview for internship.

I would like to ask this question from Preparation of Enzymes from Clarified Solution in division Enzyme Preparation and Usage of Enzyme Technology

Answer 1

Right choice is (b) Loss of cofactor or coenzymes

Easiest explanation: Loss of cofactors or coenzymes causes enzyme inactivation. The following processes doesn’t cause enzyme inactivation:

• Filtration separates simply on the basis of particle size.

• Centrifugation separates on the basis of the particle size and density difference between the liquid and solid phases.

• Crossflow microfiltration is a process in which high tangential flow of the feedstock across the surface of the filter is used.