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Immobilized __________ is prepared by entrapping Escherichia coli cells in a k-carageenan gel cross-linked with glutaraldehyde and hexamethylenediamine.

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Immobilized __________ is prepared by entrapping Escherichia coli cells in a k-carageenan gel cross-linked with glutaraldehyde and hexamethylenediamine.

(a) raffinase

(b) lactase

(c) aspartase

(d) aminoacyclase

The question was asked in an online quiz.

Query is from Immobilized Enzymes topic in portion Immobilized Enzymes and their Uses of Enzyme Technology

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Right answer is (c) aspartase

Explanation: Immobilized aspartase is prepared by entrapping Escherichia coli cells in a k – carageenan gel cross-linked with glutaraldehyde and hexamethylenediamine. Immobilized raffinase is prepared by stirring Mortierella vinacea with the sugar beet juice in a batch stirred tank reactors. Yeast lactase are immobilized by incorporation into cellulose triacetate fibers in batch-wise stirred tank reactors. Aminoacyclase is immobilised by adsorption to anion exchange resins and has an operational half-life of about 65 days at 50°C in PBRs with residence times of about 30 min.

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