Question

A crude immobilized-enzyme preparation consisting of heat -treated achromobacter liquidum cells entrapped in a polyacrylamide gel has been used to convert L-histidine to urocanic acid effectively.

(a) True

(b) False

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My enquiry is from Immobilized Enzymes in portion Immobilized Enzymes and their Uses of Enzyme Technology

Answer 1

Correct option is (a) True

Easiest explanation: Urocanic acid may be produced from L-histidine by histidase from achromobacter liquidum. The organism has urocanate hydratase activity, which removes the urocanic acid. Hence this organism cannot be used directly used. To overcome this, a brief heat treatment (70°C, 30 min) inactivates this unwanted activity but has little effect on the histidases. Hence immobilized-enzyme preparation consisting of heat – treated achromobacter liquidum cells entrapped in a polyacrylamide gel. Thus, the above statement is true.