Question

The denaturation temperature is _________

(a) 55°C

(b) 72°C

(c) 66°C

(d) 94°C

This question was posed to me in an interview.

I'm obligated to ask this question of Brewing Yeast in chapter Brewing Yeast of Fermentation Technology

Answer 1

The correct answer is (d) 94°C

To elaborate: The denaturation temperature is 94°C. It is the temperature at which the double-stranded DNA is broken down into single-stranded DNA. It is followed by the addition of primers. Two sets of primers are added: Forward primer and Reverse primer. The extension is done by Taq Polymerase at 72°C.