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Which of the following doesn’t cause enzyme inactivation?

(a) Oxidation

(b) Denaturants

(c) Filtration

(d) Irreversible inhibitors

I got this question in an interview.

This question is from Preparation of Enzymes from Clarified Solution in section Enzyme Preparation and Usage of Enzyme Technology

1 Answer

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The correct choice is (c) Filtration

The best I can explain: Filtration separates simply on the basis of particle size and does not cause enzyme inactivation. Oxidation, denaturants, irreversible inhibitors, heat, proteolysis, sub-optimal pH, loss of cofactors or coenzymes etc., causes enzyme inactivation.

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