Question

Which one of the following is not a step in production of glucose syrups using invertase?

(a) Yeast cells were autolyzed

(b) Filtration through a bed of calcium sulphate

(c) Adsorption into bone char

(d) Galactose released is destroyed by alkaline conditions

I have been asked this question at a job interview.

Asked question is from High Fructose Corn Syrups and use of Immobilized Raffinase, Invertase and Lactase topic in chapter Immobilized Enzymes and their Uses of Enzyme Technology

Answer 1

The correct answer is (d) Galactose released is destroyed by alkaline conditions

To explain I would say: The steps involved in production of glucose syrup using invertase are as follows:

• Yeast cells were autolyzed.

• Autolyzate clarified by adjustment to pH 4.7.

• Filtration through a bed of calcium sulphate.

• Adsorption into bone char which helps in decolorizing the syrups.

Galactose released is destroyed by alkaline conditions of the first stages of juice purification and does not cause any further problems while the sucrose is recovered. This is stage in removal of raffinose and not production of glucose syrups.