Question

For industrial use, debranching enzymes would be necessary.

(a) True

(b) False

I had been asked this question in an online interview.

The above asked question is from Large Scale Use of Enzyme topic in division Large Scale Use of Enzyme in Solution of Enzyme Technology

Answer 1

Answer: (a) True

Debranching enzymes are absolutely necessary for industrial use in starch processing. They are used in the enzymatic hydrolysis of starch to break down the branched polymer into glucose syrups. Without debranching enzymes, alpha-amylase alone cannot completely break down starch because it cannot cleave the alpha-1,6-glycosidic bonds at the branch points of amylopectin. The use of both alpha-amylase and debranching enzymes (like isoamylase or pullulanase) ensures complete hydrolysis and maximum glucose yield, which is economically important for industrial glucose production.