Question

Cis-acting REP 3 sequence is present in Yeast episomal plasmids. Choose the statement which holds true for it?

(a) It is present along with chromosomal centromeric sequence

(b) It is the site of action of proteins that help in partitioning

(c) It gives less stability to that of YCs

(d) The copy number is low than that of YCs

I got this question in an interview for job.

The above asked question is from Markers topic in section Using Other Organisms of Genetic Engineering

Answer 1

Correct answer is (b) It is the site of action of proteins that help in partitioning

For explanation I would say: Cis acting REP3 sequence is present in Yeast Episomal plasmids. It is a substitution for chromosomal centromeric sequence. It is having similar stability than that of YCs but a higher copy number than that of it.