Question

Strong secondary structure in a message may affect ________

(a) translation

(b) transcription

(c) both translation and transcription

(d) none of translation and transcription

I have been asked this question in an online interview.

My question is based upon Co-expression of Proteins and Optimization topic in section Use of Cloned DNA of Genetic Engineering

Answer 1

The correct answer is (a) translation

Best explanation: It is not necessary that if transcription and translation initiate well, they continue well. Factors such as secondary structure, affect translation. There are other factors such as codon usage and codon meaning which affect translation.