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Which of the following is not the function of buffer?

(a) Prevent cell lysis

(b) Chelating the metals

(c) Prevent protease action

(d) Prevent lipase action

I got this question in an internship interview.

The question is from Fermentation Laboratory topic in division Equipping a Fermentation Laboratory, the Design and Preparation of Media for Bioprocesses of Fermentation Technology

1 Answer

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Right choice is (d) Prevent lipase action

Explanation: The buffer is used to prevent the lysis of cells, and chelate the metals and prevent the action of protease enzymes. The buffer is added to the pellet prior to processing and defrosting.

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