Question

Which of the following method is useful for the isolation and detection of organisms having the ability to produce antibiotics?

(a) Crowded plate technique

(b) Auxanographic technique

(c) Enrichment Culture technique

(d) Indicator dye technique

This question was addressed to me by my school principal while I was bunking the class.

This interesting question is from Isolation and Screening of Industrially Important Microbes topic in section The Isolation, Preservation and Improvement of Industrial Microorganisms of Fermentation Technology

Answer 1

The correct answer is (a) Crowded plate technique

For explanation I would say: The Crowded Plate Technique is used for the detection and isolation of the organisms which are antibiotic producers. The serial dilutions of soil are performed and spread on a Petri plate and allowed to grow. The well-isolated colonies are antibiotic producers with the zone of inhibition.