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A test tube contains 9ml distilled water. 1g of soil is added to that test tube and the soil is allowed to settle down. Now, 1ml of that stock solution is taken and transferred to the 2nd test tube containing 9ml of distilled water. The process is repeated several times until the requirement is met. For this dilution, it may be said that the solution was diluted _______

(a) 100-fold

(b) 10-fold

(c) 1000-fold

(d) 2-fold

I had been asked this question in unit test.

I'm obligated to ask this question of Isolation and Screening of Industrially Important Microbes in portion The Isolation, Preservation and Improvement of Industrial Microorganisms of Fermentation Technology

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Right choice is (b) 10-fold

The best I can explain: The transfer of a 1ml solution to 9ml d/w is called as serial 10-fold dilution. It is used to reduce the dense culture of cells to usable concentration.

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