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Which of the following method proceeds with 2-plate preparation?

(a) Crowded plate technique

(b) Auxanographic technique

(c) Enrichment Culture technique

(d) Indicator dye technique

The question was posed to me by my college director while I was bunking the class.

I want to ask this question from Isolation and Screening of Industrially Important Microbes in portion The Isolation, Preservation and Improvement of Industrial Microorganisms of Fermentation Technology

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Best answer
The correct choice is (b) Auxanographic technique

The best explanation: Auxanographic technique proceeds with 2-plate preparation. The 1^st plate is used to isolate and detect prototrophs whereas 2^nd plate is used for identification of auxotrophs.

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