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Which of the following method is useful for isolation and detection of organisms having the ability to produce growth factors?

(a) Crowded plate technique

(b) Auxanographic technique

(c) Enrichment Culture technique

(d) Indicator dye technique

This question was posed to me by my college director while I was bunking the class.

I want to ask this question from Isolation and Screening of Industrially Important Microbes in section The Isolation, Preservation and Improvement of Industrial Microorganisms of Fermentation Technology

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The correct answer is (b) Auxanographic technique

For explanation: The Auxanographic technique is used for the detection and isolation of the organisms which are growth factor producers. The growth factor-like amino acids, vitamins promotes the growth of auxotrophic mutants.

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