Question

Which of the following method is useful for isolation and detection of organisms having the ability to produce organic acids?

(a) Crowded plate technique

(b) Auxanographic technique

(c) Enrichment Culture technique

(d) Indicator dye technique

The question was asked during an internship interview.

The doubt is from Isolation and Screening of Industrially Important Microbes in division The Isolation, Preservation and Improvement of Industrial Microorganisms of Fermentation Technology

Answer 1

Correct answer is (d) Indicator dye technique

The best explanation: The use of Indicator Dye Technique is used for detection and isolation of the organisms which are organic acid producers. The organic acid producers produce acid which changes the colour of media and is thus detected easily.