Question

Capping can be introduced by the use of cap analogue. Which of the statement is true?

(a) Cap analogue can be introduced at the end of the transcript

(b) Cap analogue can be introduced at the start of the transcript

(c) Cap analogue can be introduced anywhere in the transcript

(d) Cap analogue can be introduced both at the end and starting of the transcript

I had been asked this question in an interview.

Asked question is from Synthesis of RNA in portion Use of Cloned DNA of Genetic Engineering

Answer 1

Right option is (b) Cap analogue can be introduced at the start of the transcript

Best explanation: For achieving capping, cap analogue should be introduced. Cap analogue should be introduced at the starting of the transcript. It is so because it requires 5’ end.