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Which of the following is the most traditional of the ‘traditional methods’ of strain characterization?

(a) Genetic fingerprinting

(b) Assimilation

(c) Flocculation test

(d) Giant colony method

The question was posed to me during an online exam.

I'd like to ask this question from Brewing Yeast in section Brewing Yeast of Fermentation Technology

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The correct option is (d) Giant colony method

To elaborate: Giant colony method is the most traditional method of the traditional methods of strain characterization. Lindner reported that up to ten single colonies on thick wort-gelatin plates give rise to ‘giant colonies’ when incubated for three weeks at 18°C.

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