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Which of the following is used to separate molecules based on affinity?

(a) Column chromatography

(b) Ion exchange chromatography

(c) Thin layer chromatography

(d) Affinity chromatography

I had been asked this question during an online exam.

I would like to ask this question from Fermentation Products Recovery and Purification topic in portion Recovery and Purification of Fermentation Products, Effluent Treatment, Bioreactor Design Considerations of Fermentation Technology

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Right option is (d) Affinity chromatography

The explanation: The affinity chromatography is used in the separation of biological molecules based on their affinity with a particular substance. The molecules with low affinity with the stationary phase are eluted first and those with high affinity are recovered later.

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