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Which of the following substrate is not used by methanotrophic bacteria to oxidize methane?

(a) Methanol

(b) Formaldehyde

(c) Methane

(d) Carbon dioxide

I have been asked this question during a job interview.

Enquiry is from Cometabolic Degradation of Organic Pollutants topic in section Biodegradation of Organic Pollutants of Environmental Biotechnology

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The correct answer is (d) Carbon dioxide

The explanation: Methanotrophs or methanophiles are prokaryotes that can metabolise and convert the methane into by-products by using it as their only source of carbon and energy. Methanotrophic bacteria to oxidize methane to carbon dioxide via Methanol, Formaldehyde, Methane and not carbon dioxide itself.

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