Question

In which of the following systems, transcription and translation are carried out together?

(a) Reticuloycte lysate

(b) Wheat gram extract

(c) Both in reticulocyte lysate and wheat gram extract

(d) S-30 extract

The question was asked in a national level competition.

My question is taken from Synthesis of Proteins and Translation In Vitro topic in section Use of Cloned DNA of Genetic Engineering

Answer 1

Right choice is (d) S-30 extract

To elaborate: S-30 extract is the one in which transcription and translation are carried out together. In wheat gram extract and reticulocyte lysate, they are carried out separately.