Question

The Replica plate technique was used for _________

(a) Isolation of auxotrophs

(b) Isolation of revertants

(c) Isolation of analogue-resistant mutants

(d) Isolation of prototrophs

This question was posed to me in final exam.

Origin of the question is Improvement of Industrial Microorganisms topic in portion The Isolation, Preservation and Improvement of Industrial Microorganisms of Fermentation Technology

Answer 1

The correct answer is (b) Isolation of revertants

For explanation I would say: The Replica plate technique was used for the isolation of revertants. The isolation of auxotrophs and prototrophs proceeds with auxanographic technique and the isolation of analogue-resistant mutants is done by Gradient Plate technique.