Question

If transfer of DNA from Agrobacterium to plants is done via incubation of explanted material and the vector containing DNA of interest and then selection is done via selectable marker then this method is called as _________

(a) transformation

(b) co-cultivation

(c) co-transformation

(d) floral dipping

I have been asked this question by my college professor while I was bunking the class.

Query is from Vascular Plants in chapter Using Other Organisms of Genetic Engineering

Answer 1

Correct answer is (b) co-cultivation

The explanation: Co-cultivation is the method of incubation of explanted material and the vector containing DNA of interest. Further selection is done via selectable marker such as antibiotic resistance. Floral dipping is an approach used for plant Arabidopsis.