Question

Cells mutant in _____ gene can be selected by using 5-fluoro orotic acid, which is turned into ____ products by wild type protein.

(a) SUP4, toxic

(b) SUP4, non-toxic

(c) URA3, toxic

(d) URA3, non-toxic

The question was asked by my school principal while I was bunking the class.

Enquiry is from Markers topic in chapter Using Other Organisms of Genetic Engineering

Answer 1

The correct answer is (c) URA3, toxic

The best explanation: Cells mutant in URA3 gene can be selected by using 5-fluoro orotic acid, which is turned into toxic products by wild type protein. URA3 gene is responsible for uracil biosynthesis.