Question

What is use of density gradient centrifugation?

(a) To purify viruses, ribosomes, membranes

(b) Toremovedirt

(c) To remove fine particles

(d) To remove large particles

I got this question in class test.

I want to ask this question from Mechanical Separation in chapter Mechanical Separation of Food Processing Unit Operations

Answer 1

The correct option is (a) To purify viruses, ribosomes, membranes

Easiest explanation: Density gradient centrifugation is used to purify viruses, ribosomes, membranes. A sucrose density gradient is created by gently overlaying lower concentrations of sucrose on higher concentrations in centrifuge tubes where particles of interest are placed on top in ultracentrifuges. The particles move through the gradient until they reach the same sucrose density and fraction is removed and analyzed.