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Can negative peaks occur in HPLC?

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+1 vote
in Food Processing Unit Operations by (110k points)
Can negative peaks occur in HPLC?

(a) True

(b) False

I had been asked this question in an internship interview.

Question is taken from Absorption Mechanisms topic in section Absorption of Food Processing Unit Operations

1 Answer

+2 votes
by (736k points)
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Best answer
The correct choice is (a) True

The explanation is: A negative peak means that there is less absorbance while the peak is passing through the detector than when the mobile phase is passing through. It is due to the mobile phase which has more absorbance than the analyte at the monitored wavelength.

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