+1 vote
in Food Processing Unit Operations by (110k points)
Why acetonitrile is used as solvent in HPLC?

(a) Improve chromatographic peak shape

(b) Provide a source of protons in reverse phase LC/MS

(c) Provide a source of electrons in reverse phase LC/MS

(d) Improve chromatographic peak shape and provide a source of protons in reverse phase LC/MS

The question was posed to me in semester exam.

This interesting question is from Absorption Mechanisms topic in chapter Absorption of Food Processing Unit Operations

1 Answer

+1 vote
by (736k points)
selected by
 
Best answer
Correct answer is (d) Improve chromatographic peak shape and provide a source of protons in reverse phase LC/MS

The best I can explain: In High pressure liquid chromatography, the B solvent used is HPLC grade organic solvent like acetonitrile, methanol with 0.1% acid. This acid is used for improving the shape of chromatographic peak and to provide source of protons in the reverse phase LC/MS.

Related questions

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community
...