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In which of the following methods, the intensity of emitted light is used to study enzyme reaction?

(a) Discontinuous assay

(b) Luminescence method

(c) Biosensors

(d) Spectrophotometer

I had been asked this question during an interview.

My enquiry is from Enzyme Assay Techniques topic in portion Enzyme Assay & Enzyme Catalysis of Enzyme Technology

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The correct option is (c) Biosensors

Easy explanation: In Luminescence method, the intensity of emitted light is used to study enzyme reaction. In discontinuous assay, there is a “stop and sample” procedure which is used. The analytical devices which converts a biological response into an electrical signal is referred to as biosensors. The change in absorbance is used as basis for assaying enzymes using spectrophotometer.

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